This study reveals that bats in the United States can harbor diverse trypanosome parasites, including the first reported case of Trypanosoma cruzi (the Chagas disease agent) in a US bat and the first North American detection of Trypanosoma dionisii. Researchers tested 593 bats across Texas, finding one bat (0.17%) positive for T. cruzi carrying the TcI strain associated with human disease, nine bats (1.5%) with T. dionisii showing three distinct variants across three bat species, and five bats (0.8%) with Blastocrithidia spp., marking the first detection of these insect trypanosomes in mammals. These findings highlight the potential role of migratory bats in transcontinental movement and maintenance of disease-causing parasites.