Toxoplasma gondii is a zoonotic pathogen of warm-blooded hosts, meaning it can infect humans. Seroprevalence in wildlife is often assessed using cross-sectional data, but few studies have tracked infections within individuals over time. We present a 4-year dataset from white-tailed deer with repeated sampling over time. We observed seroconversion from seronegative to seropositive within individuals, as expected. Accurate assignment of serostatus is needed for evaluating the effects of infection. We discuss implications for assigning serostatus for chronic toxoplasmosis infection for 3 scenarios.